The Epi肠 tissue model (SMI-100) offers numerous advantages versus currently available preclinical testing models such as Caco-2. 基于人体细胞的3D模型包含肠细胞, paneth细胞, M细胞, 簇状细胞和肠干细胞分化成高度分化的细胞, 极化上皮. The Epi肠FT tissue (SMI-100-FT) includes an underlying lamina propria containing normal human intestinal fibroblasts and is useful to study inflammation, 伤口修复, 先天免疫反应, 和纤维化. Both tissues are cultured at the air-liquid interface (ALI) to allow physiological (luminal) exposure conditions. Epi肠 recapitulates many aspects of normal intestinal function including barrier, 新陈代谢, 炎症和毒性反应, 类似于人类肠道组织.
Important features of Epi肠 include the presence of functional tight junctions and brush borders at the apical tissue surface. 在活的有机体内, brush borders are in direct contact with luminal contents and include at least 22 metabolic enzymes and 19 drug transporters. Epi肠 tight junctions and brush borders closely mimic human structure and physiology.
The Epi肠 3D human tissue model is routinely utilized for screening pharmaceuticals for a range of endpoints including GI toxicity and inflammation, 药物代谢, 药物渗透, 纤维化, 和肠道感染. Straightforward protocols and the evaluation of early molecular and cellular endpoints often allow scientists to acquire data in days, 而不是几周或几个月.
胃肠道毒性
Use Epi肠 for a highly predictive assay to evaluate GI toxicity of pharmaceuticals.
Epi肠 (SMI-100) Transepithelial Electrical Resistance (TEER) Protocol
炎症和纤维化
Utilize Epi肠’s highly controlled 在体外 培养条件 to study intestinal inflammation, 伤口修复, 和纤维化, 评估治疗效果.
药物输送 & 新陈代谢
Utilize Epi肠 for highly predictive and human-relevant drug delivery. 评估原料药生物利用度(内流)和外排转运, 药物代谢, 和药物之间的相互作用. 表肠可与其他生理系统共培养.g. liver cells) in microfluidic systems to examine the effect of drug metabolites on GI toxicity or to predict 药物渗透/absorption via the “luminal” side of the tissue.
伤口修复/上皮归还
Use Epi肠FT to better understand mechanisms of wound healing in the gut and to evaluate therapeutic effects.
肠道感染
利用外肠研究胃肠道感染, host-pathogen交互, 先天免疫反应.
浏览我们的 参考图书馆 to learn more about how researchers have used our Epi肠 tissue in these areas of study.
组织:
工具: SMI-100和SMI-100- ft Epi肠试剂盒由24个个体组织组成. SMI-196 or SMI-196-FT Epi肠 kits consist of 96 tissues in a high throughput screening plate. Each kit contains tissues, culture medium to support experiments for 24 hours, and plasticware. 请联系新利app全站具体的套件内容.
格式:
- SMI-100 and SMI-100-FT: Individual tissues cultured in cell culture inserts.尺寸:9毫米组织直径(表面积= 0.6 cm2 )，下层微孔膜孔径= 0.4 μm
- SMI-196 and SMI-196-FT: Tissues grown in 96-well high throughput screening plate. 尺寸:0.组织直径19厘米(表面积= 0.11 cm2). 底层微孔膜孔径=0.4 μm
文化: 气液界面
很多数字: 每周生产的组织批号都有一个特定的批号. A letter of the alphabet is appended to the end of the lot number to differentiate between individual sets of 24 tissues within a given production lot of tissues. 在细胞方面，生产批量内的所有组织包都是相同的, medium, 处理, 培养条件, 等.
装运: 4°C，培养基中添加琼脂糖凝胶
装运: 每星期一
交付: 周二上午，通过联邦快递优先服务(美国). 美国以外地区:周二至周三，视地点而定.
保质期: Including time in transit, tissues may be stored at 4°C for up to 2 days prior to use. 然而, the best reproducibility will be obtained if tissues are used consistently on the same day, e.g. Tuesday afternoon or following overnight storage at 4°C (Wednesday morning).
长度的实验: Tissue cultures can be continued for ONE (1) MONTH or more with good retention of normal morphology. 面巾纸必须每隔一天用5.0ml维护介质(SMI-100-MM). Cell culture inserts are placed atop washers (EPI-WSHR) or culture stands (MEL-STND) in 6-well plates to allow use of 5.0 ml.
细胞:
类型: 原代人小肠上皮细胞(HSIE)
基因组成: 单一捐赠者
来自: 健康的成年供体
选择: 可根据要求提供替代捐助者
筛选: 艾滋病毒，乙型肝炎，丙型肝炎，支原体
介质:
基础培养基: 杜尔贝科改良Eagle中型(DMEM)
生长因子/激素: 表皮生长因子及其他专有因子
抗生素: 庆大霉素5µg / ml
抗真菌剂: 两性霉素B 0.25µg / ml
酸碱指示剂: 酚红
其他添加剂: 专有的
选择: 酚红-free, antibiotic-free, anti-fungal-free medium and tissues are available. 代理至少在发货前3天被移除.
质量控制和无菌:
目视检查: All tissues are visually inspected and if physical imperfections are noted, 组织被拒绝装运
不育: 在整个生产过程中使用的所有介质都要进行无菌检查. Maintenance medium is incubated with and without antibiotics for 1 week and checked for sterility. The agarose gel from the 24-well plate used for shipping is also incubated for 1 week and checked for any sign of contamination
筛查病原体: All cells are screened and are negative for HIV, hepatitis B and hepatitis C using PCR. 然而, no known test method can offer complete assurance that the cells are pathogen free. 因此, these products and all human derived products should be handled at BSL-2 levels (biosafety level 2) or higher as recommended in the CDC-NIH manual, “微生物和生物医学实验室的生物安全,” 1998. For further assistance, please contact your site Safety Officer or 新利app全站 technical service
地段失败的通知: 如果一个组织批次的质量控制或无菌检测不合格, the customer will be notified and the tissues will be replaced without charge. 因为我们的QC和无菌测试是在出货后进行的, 在正常情况下，我们会尽快通知你, ET-50失败将在周四下午5点前通知.m.; sterility failures will be notified within 8 days of shipment)
